Irish Researchers Call for Better Understanding of Bacterial Toxins

Further research into this group of toxins will lead to the identification of novel targets for antibiotic and vaccine development for the treatment and prevention of human disease.

Aug 29 2011 --- A better understanding of how bacterial toxins cause common human diseases may lead to their improved treatment and prevention according to a paper just published by Irish and US scientists in Nature Reviews Microbiology.

Scientists discuss the identification, genetics and biochemistry of streptolysin S (SLS), a bacterial toxin produced by the bacteria Streptococcus pyogenes. S. pyogenes causes a wide variety of infections of the upper respiratory tract and the skin, with complications leading to invasive diseases such as the “flesh-eating” skin disease, necrotising fasciitis, and streptococcal toxic shock syndrome. As a consequence of these diseases, and other auto-immune complications like acute rheumatic fever, and subsequent rheumatic heart disease, up to half a million deaths per year worldwide are attributed to S. pyogenes infections.

Further research into this group of toxins will lead to the identification of novel targets for antibiotic and vaccine development for the treatment and prevention of human disease.

Lead author of the review, Evelyn M Molloy is a PhD student in microbiology at the Alimentary Pharmabiotic Centre, UCC, under the supervision of Paul Cotter, Colin Hill and Paul Ross. The research involves collaboration between the researchers at the Alimentary Pharmabiotic Centre based in UCC and Teagasc Moorepark Food Research Centre, along with colleagues at the University of Illinois at Urbana-Champaign, USA.

Molloy’s interest in listeriolysin S, a member of the SLS toxin family found in the food poisoning bacteria Listeria monocytogenes led to a collaboration with Douglas Mitchell at the University of Illinois at Urbana-Champaign, USA. Research trips by Molloy to the University of Illinois were funded by the Science Foundation Ireland and the Society for General Microbiology. Molloy’s research is on food-grade antimicrobial peptides (‘bacteriocins’), which can be employed to enhance food safety and improve human and animal health.

Infections likely to have been caused by S. pyogenes have been documented in humans for many centuries, including the apparent scarlet fever epidemic described by Hippocrates in the fifth century BC. Pasteur was the first to report isolation of this organism from the bloodstream in a woman with puerperal sepsis in 1884. The ability of the SLS toxin to destruct red blood cells was first discovered in the 1930s and since then its contribution to S. pyogenes infection has been the subject of much attention. However, despite a 100-year history of research, it has only recently been established that there is a family of SLS toxins produced by other streptococci and food poisoning bacteria such as L. monocytogenes and Clostridium botulinum.