Default values used in risk assessments should always be scientifically justified and be based on existing data representing average (typical) values for the missing information. The guidance focuses on the most commonly used default values by EFSA’s Scientific Panels, Scientific Committee and Units. It identifies harmonized default values for body weight, total liquid intake, a number of uncertainty factors used in establishing health-based guidance values, and factors for converting chemical substance concentrations in feed or drinking water to doses utilized in experimental animal studies to assess toxicity of the substances.